Asian Journal of Physics Vol 32, Nos 1 & 2 (2023) 5-10

Application of Raman spectroscopy in the analysis of plant tissues

Hartwig Schulz
Consulting & Project Management for Medicinal &
Aromatic Plants Waltraudstrasse 4, 14532 Stahnsdorf, Germany

On February 28, 1928, at the Indian Association for the Cultivation of Sciences (IACS), Kolkata (India), C V Raman and his associates for the first time experimentally showed that when monochromatic light is scattered by transparent media, the scattered light contains not only the original colour, but also other colours. This effect was named as Raman effect [1] and it belongs to one of the four most important discoveries in physics with the highest impact to applications in modern life [2].
My first contact with vibrational spectroscopy, (which employs IR and Raman Spectroscopy), was during my Ph D thesis, where I used IR and Raman spectroscopy to identify newly synthesised geometric isomers of various complex substances of the type OsXnY4–n ox (X,Y= Cl, Br, J; n = 1, 2, 3). The symmetry-related differences of the individual isomers were mainly evident in the Os-halogen vibrational region. According to the selection rules, predominantly symmetrical vibrations of the molecules could be observed in the Raman spectrum and assigned to the individual vibrational modes.
© Anita Publications. All rights reserved.

Peer Review Information
Method: Single- anonymous; Screened for Plagiarism? Yes
Buy this Article in Print © Anita Publications. All rights reserve


  1. Singh R, Rastogi V K, On the Occasion of 90th Anniversary of the Raman Effect, Asian J Phys, 27(2018) 93–101.
  2. .Rastogi V K, An Interview with Wolfgang Kiefer, On the occasion of 80th birthday of Wolfgang; Feb 12, 2021, Asian J Phys, 30(2021)239–250.
  3. Baranska M, Schulz H, Application of infrared and Raman spectroscopy for analysis of selected medicinal and spice plants, Z Arzn Gew Pfl, 2(2006)72–80.
  4. Bukowska J, Piotrowski P, Surface-enhanced Raman Scattering (SERS) in Bioscience: A Review of Application, in: Optical Spectroscopy and Computational Methods in Biology and Medicine, editor: Malgorzata Baranska, (Springer, Dordrecht), (2014), pp. 29-59.
  5. Fiedler A, Baranska M, Schulz H, FT-Raman spectroscopy—a rapid and reliable quantification protocol for the determination of natural indigo dye in Polygonum tinctorium, J Raman Spectrosc, 42(2011)551–557.
  6. Baranska M, Schulz H, Determination of Alkaloids through Infrared and Raman Spectroscopy, in: The Alkaloids, Vol 67, editor: Cordell G A, (Elsevier), 2009, pp 217–255.
  7. Schulz H, Baranska M, Identification and quantification of valuable plant substances by IR and Raman spectroscopy, Vib Spectrosc, 43(2007)13–25.
  8. Stehle M A, Rösch P, Baranska M, Schulz H, Popp J, On the way to a quality control of the essential oil of fennel by means of Raman spectroscopy, Biopolymers, 77(2005)44–52.
  9. Baranska M, Schulz H, Reitzenstein S, Uhlemann U, Strehle M A, Krüger H, Quilitzsch R, Foley W, Popp J, Biopolymers, 78(2005)237–248.
  10. Baranska M, Schulz H, Baranski R, Nothnagel T, Christensen L P, In situ simultaneous analysis of polyacetylenes, carotenoids and polysaccharides in carrot roots, J Agric Food Chem, 53(2005)6565–6571.
  11. Schulz H, Baranska M, Baranski R, Potential of NIR-FT-Raman spectroscopy in natural carotenoid analysis, Biopolymers, 77(2005)212–221.
  12. Gamsjaeger S, Baranska M, Schulz H, Heiselmayer P, Musso M, Discrimination of carotenoid and flavonoid content in petals of pansy cultivars (Viola x wittrockiana) by FT-Raman spectroscopy, J Raman Spectrosc, 42 (2011)1240–1247.