Control of selective two-photon fluorescence suppression by one-color pulse-pair excitation

Debjit Roy¹, Arijit Kumar De² and Debabrata Goswami³

Abstract

For studying the selective excitation of a particular fluorophore in fluorescence microscopy, the effect of the laser pulse chosen is immense. To study the ramifications of this selection, we present a thorough study of one-color stimulated emission depletion of the two-photon fluorescence of selective fluorophores by changing the wavelength and delay between two identical laser pulses. © Anita Publications. All rights reserved.
Keywords: Two-photon fluorescence, Stimulated emission, Pulse-pair excitation, One-color scheme, Multiphoton microscopy

Peer Review Information
Method: Single- anonymous; Screened for Plagiarism? Yes
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