Application of Raman spectroscopy in the analysis of plant tissues

Hartwig Schulz
Consulting & Project Management for Medicinal &
Aromatic Plants Waltraudstrasse 4, 14532 Stahnsdorf, Germany

On February 28, 1928, at the Indian Association for the Cultivation of Sciences (IACS), Kolkata (India), C V Raman and his associates for the first time experimentally showed that when monochromatic light is scattered by transparent media, the scattered light contains not only the original colour, but also other colours. This effect was named as Raman effect [1] and it belongs to one of the four most important discoveries in physics with the highest impact to applications in modern life [2].
My first contact with vibrational spectroscopy, (which employs IR and Raman Spectroscopy), was during my Ph D thesis, where I used IR and Raman spectroscopy to identify newly synthesised geometric isomers of various complex substances of the type OsXnY4–n ox (X,Y= Cl, Br, J; n = 1, 2, 3). The symmetry-related differences of the individual isomers were mainly evident in the Os-halogen vibrational region. According to the selection rules, predominantly symmetrical vibrations of the molecules could be observed in the Raman spectrum and assigned to the individual vibrational modes.
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References

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